q5 polymerase neb cat (New England Biolabs)

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New England Biolabs q5 polymerase neb cat
Q5 Polymerase Neb Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33799 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t4+dna+ligase+neb+cat/T4+DNA+Ligase/pm42822443-233-135-137
Average 99 stars, based on 33799 article reviews

q5 polymerase neb cat - by Bioz Stars, 2026-10

99/100 stars

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Recombinant:

Article Title: SEPHS2 loss reprograms cancer metabolism from oxidative phosphorylation to gluconeogenesis via PCK1 stabilization.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies SEPHS2 proteintech Cat#14109-1-AP; Lot#00124159; RRID: AB_2878014 LA/LP Polyclonal antibody(SEPSECS) proteintech Cat#11551-1-AP; Lot#00121618; RRID:AB_2186853 PCK1 (D12F5) Rabbit mAb Cell Signaling Technology Cat#12940S; Lot#3; RRID:AB_2687968 Anti FLAG Nanobody Agarose Beads ALPALIFEBIO Cat#KTSM1308; Lot#240810; RRID:AB_3740166 Anti-HA tag Rabbit mAb PTM bio Cat#PTM-5389; Lot#RL110916; RRID:AB_3740293 Anti-DYKDDDDK Tag Mouse mAb PTM bio Cat#PTM-6075; Lot#MN120818; RRID:AB_3740294 Pan-Acetyl-Lysine Mab Abmart Cat#M30069S; Lot#334095; RRID: AB_2936273 β-actin Beta Actin Monoclonal antibody proteintech Cat#66009-1-Ig; Lot#10052137; RRID: AB_2687938 HRP-conjugated GAPDH Monoclonal antibody proteintech Cat#HRP-60004; Lot#21020050; RRID: AB_2107436 HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L) proteintech Cat#SA00001-2; Lot#20001102; RRID: AB_2722564 HRP-conjugated Affinipure Goat Anti-Mouse IgG(H + L) proteintech Cat#SA00001-1; Lot#20001336; RRID: AB_2722565 Ki-67 Invitrogen Cat#MA5-14520; Lot#ZC4251801; RRID: AB_10979488 BrdU HUA BIO Cat#HA721283; Lot#H651616016; RRID: AB_3072400 Chemicals, peptides, and recombinant proteins DMEM VivaCell Cat#C3113-0500; Lot#2523153 Minimum Essential Medium Eagle,(MEM-E) VivaCell Cat#C3050-0500; Lot#2421230 glucose-free DMEM Wisent Cat#319-061-CL; Lot#319061013 13C6-glucose Cambridge Isotope Laboratories Cat#CLM-1396-1; Lot#PR-34213 Fetal bovine serum Excell Cat#FSP500; Lot#12C019 Penicillin-Streptomycin Solution, 100X Beyotime Cat#C0222; Lot#A326250724 Insulin procell Cat#PB180432; Lot#WH1724D184 Trypsin-EDTA Solution with phenol red Beyotime Cat#C0204; Lot#Z985241103 Hieff Trans® Liposomal 2000 Transfection Reagent Yeasen Cat#40802ES03; Lot#H3217980 Puromycin Dihydrochloride Beyotime Cat#ST551-50mg; Lot#030424240716 Blasticidin S, Sterile MCE Cat#HY-K1054; Lot#676494 PEI transfection reagent Yeasen Cat#40815ES03; Lot#H5528320 opti-mem Gibco Cat#31985-070; Lot#3141616 Annealing Buffer for DNA Oligos (5X) Beyotime Cat#D0251; Lot#090721220401 AgeI-HF NEB Cat#R3552S; Lot#10126565 EcoRI-HF NEB Cat#R3101S; Lot#10146052 BsmBI-v2 NEB Cat#R0739S; Lot#10170454 T4 DNA Ligase NEB Cat#M0202T; Lot#10126210 Ampicillin Beyotime Cat#ST007; Lot#060221220104 UK-5099 MCE Cat#HY-15475; Lot#07552 (Continued on next page) Cell Reports 45, 117297, May 26, 2026 15 ..

Article Title: Phage induction of Staphylococcus aureus pathogenicity islands promotes the CRISPR-Cas adaptive immune response.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains S. aureus RN4220 Kreiswirth et al. 75 N/A S. aureus RN10616 Ubeda et al. 33 N/A S. aureus RN10628 Ubeda et al. 33 N/A S. aureus OS2 Schneewind et al. 76 N/A S. aureus ST16 Ubeda et al. 33 N/A S. aureus ST24 Ubeda et al. 33 N/A S. aureus ST126 Damle et al. 77 N/A S. aureus TB4 Bae et al. 41 N/A S. aureus DVB3 This study N/A S. aureus DVB6 This study N/A S. aureus DVB7 This study N/A S. aureus DVB8 This study N/A S. aureus DVB11 This study N/A S. aureus DVB16 This study N/A S. aureus DVB21 This study N/A Φ80α-vir Banh et al. 42 N/A Φ80α-vir SaPI1-escaper #1 This study N/A Φ80α-vir(21 seed ) This study N/A Φ80α-vir(21 PAM ) This study N/A Chemicals, peptides, and recombinant proteins Brain-Heart Infusion (BHI) Agar Becton Dickson Cat#241810 Brain-Heart Infusion (BHI) Broth Becton Dickson Cat#238400 Chloramphenicol GoldBio Cat#C-105-100 Erythromycin GoldBio Cat#E− 350-100 Tetracycline Hydrochloride GoldBio Cat#T-101-100 Calcium Chloride Dihydrate (White Crystals to Powder), Fisher BioReagents Fisher Scientific Cat#BP510-500 Glycerol (Certified ACS), Fisher Chemical Fisher Scientific Cat#G33-500 T4 Polynucleotide Kinase NEB Cat#M0201S T4 DNA Ligase NEB CatM0202M T4 DNA Ligase Reaction Buffer NEB Cat#B0202S BsaI-HF NEB Cat#R3535L Mitomycin C AG Scientific Cat#M-1108 Phusion High-Fidelity DNA Polymerase Thermo Fisher Scientific Cat#F530L Applied Biosystems Fast SYBR Green Master Mix Thermo Fisher Scientific Cat#4385612 NEBuilder HiFi DNA Assembly Master Mix NEB Cat#2621L Lysostaphin AMBI Products Cat#LSPN-50 Critical commercial assays MinElute PCR Purification Kit QIAGEN Cat#28004 QIAprep Spin Miniprep Kit QIAGEN Cat#27104 QIAquick Gel Extraction Kit QIAGEN Cat#28704 DNeasy Blood & Tissue Kit QIAGEN Cat#69504 (Continued on next page) Cell Reports 45, 116776, January 27, 2026 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Wizard Genomic DNA Purification Kit Promega Cat#A1125 Qubit 1X dsDNA HS Assay Kit (Invitrogen) Thermo Fisher Scientific Cat#Q33231 High Sensitivity D1000 ScreenTape Agilent Cat#5067-5584 High Sensitivity D1000 Reagents Agilent Cat#5067-5585 TruSeq Nano DNA LT Library Prep Kit Illumina Cat#20015964 MiSeq Reagent Kit v2 (300-cycles) Illumina Cat#MS-102-2002 Deposited data Next-generation sequencing files (Illumina NGS) Sequence Read Archives (SRA) BioProject accession: PRJNA1357993 Oligonucleotides See ‘‘Oligonucleotide primers used in this study’’ in Table S5 for nucleotide sequences N/A Recombinant DNA See ‘‘Plasmids used in this study’’ in Table S4 for construction details N/A pC194 Horinouchi et al. 46 N/A pDVB08 Banh et al. 42 N/A pDVB47, designated pCRISPR-II(spcNT) This study N/A pDVB52, designated pCRISPR-II(spc21) This study N/A pDVB55, designated pCRISPR-II(spc14) This study N/A pDVB59, designated pCRISPR-III(spcNT, dcsm6) This study N/A pDVB61, designated pCRISPRIII(spc14,dcsm6) This study N/A pDVB96, designated pCRISPRII(spc21,spc05) This study N/A pDVB99, designated pCRISPR-II This study N/A pDVB119, designated pCRISPR-II(Δcas1) This study N/A pGG-BsaI-R, designated pCRISPRIII(spcNT) Jiang et al. 28 N/A pWJ191, designated pCRISPR-III(spc47) Jiang et al. 28 N/A pWJ241, designated pCRISPRIII(spc47,dcsm6) Jiang et al. 28 N/A Software and algorithms Photoshop CC Adobe https://www.adobe.com/products/ photoshop.html Illustrator CC Adobe https://www.adobe.com/products/ illustrator.html SnapGene Insightful Science https://www.snapgene.com Python v3.8 Python Software Foundation https://www.python.org/downloads/ release/python-380/ PyCharm CE JetBrains https://www.jetbrains.com/pycharm/ download/#section=mac GraphPad Prism 10 Insightful Science https://www.graphpad.com/scientific- software/prism/ Custom code used to analyze NGS data by converting.sam files into.wig files This study https://github.com/Marraffini-Lab/Banh_ etal_2025 Other Infinite 200 PRO Plate Reader TECAN N/A MiSeq System Illumina N/A S220 Focused-Ultrasonicator Covaris N/A S-Series Holder microTUBE Covaris Cat#500114 14 Cell Reports 45, 116776, January 27, 2026

Article Title: Repeated emergence of giant microRNA hairpins across invertebrates.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains TOP10 Chemically Competent E. coli Our lab N/A E.coli DH5α cells ThermoFisher Scientific Cat#18258-012 E.coli DH10Bac cells ThermoFisher Scientific Cat#12033-015 Biological samples Heliconius melpomene pupal tissue Arnaud Martin N/A Daphnia magna genomic DNA Marcin Dziuba and Meghan Duffy; Lev Yampolsky N/A Chemicals, peptides, and recombinant proteins ATP, [γ-P32] PerkinElmer Cat#BLU502Z250UC UTP, [α-P32] PerkinElmer Cat#BLU007H250UC Penicillin-Streptomycin Life Technologies Cat#15070063 Trizol reagent Life Technologies Cat#15596018 Lipofectamine 2000 Thermo Fisher Cat#11668030 T4 Polynucleotide Kinase NEB Cat#M0201L T4 DNA Ligase NEB CatM0202L Gel Loading Buffer II Invitrogen Cat#AM8547 Strep-Tactin Superflow high-capacity resin IBA Lifesciences Cat# 2-1208-025 5-aminolevulinic acid hydrochloride Sigma Cat#A378505G d-Desthiobiotin Millipore-Sigma Cat#D1411-1G RNase-free DNase I NEB Cat#M0303S RNase-free water Sigma Cat#W3513 Amicon Ultra centrifugal filter (30kDa MWCO) Millipore-Sigma Cat#903096 Proteinase K NEB Cat#p8107S SYBR Gold Nucleic acid gel stain ThermoFisher Scientific Cat#S11494 HiTrap SP-HP Column Cytiva Cat#17115201 Superose 6 increase 10/300 column Cytiva Cat#29091596 Opti-MEM Reduced Serum Medium Thermo Fisher Cat#51985034 HyClone CCM3 media Cytiva Cat#SH30065.02 ESF921 cell culture media Expression Systems Cat#96-001-01 Critical commercial assays Dual Glo luciferase assay system Promega Cat#E2940 MEGAscript TM T7 Transcription Kit Thermo Fisher Cat# AM1334 Decade marker system Thermo Fisher Cat#AM7778 Phusion TM High-Fidelity DNA Polymerase NEB Cat# M0530 SequaGel UreaGel System National Diagnostics Cat#EC-833 Experimental models: Cell lines HEK293T cells Our lab N/A S2R + cells Our lab N/A Sf9 insect cells ThermoFisher Scientific Cat#11496015 High Five insect cells ThermoFisher Scientific Cat#B855502 Oligonucleotides Oligonucleotides for plasmid construction, see Table S7 This study N/A (Continued on next page) 18 Cell Reports 44, 116243, September 23, 2025 ..

Article Title: Functional assessment of all ATM SNVs using prime editing and deep learning.
Article Snippet: Resource Functional assessment of all ATM SNVs using prime editing and deep learning

Article Title: An AI-driven workflow for the accelerated optimization of cell-free protein synthesis
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies anti-6xHistidin-tag Sigma Cat#SAB2702220; RRID: AB_3095553 anti-GFP-tag Cell signaling Cat#2555; RRID: AB_10692764 anti-HiBiT-tag Promega Cat#N720A Bacterial and virus strains Escherichia coli DH5α Lab stock Escherichia coli BL21 (DE3) Lab stock Escherichia coli W3110 Lab stock Biological samples E. coli cell-free lysates Synthelis Biotech HeLa Lysate ThermoScientific Cat#88882 Chemicals, peptides, and recombinant proteins HEPES Sigma-Aldrich Cat#H3375 Nucleotide mix - Adenosine 5-triphosphate dipotassium salt hydrate Sigma-Aldrich Cat#A8937 Nucleotide mix - Guanosine 5ʹ-Triphosphate, Disodium Salt Sigma-Aldrich Cat#371701 Nucleotide mix - Sel disodique cytidine-5 ′ -triphosphate, 98+ % Thermo Scientific Chemicals Cat#J62238.ME Nucleotide mix - Uridine 5 ′ -triphosphate, sel trisodique, hydraté , 90% Thermo Scientific Chemicals Cat#226310010 3- PGA (Acide D-(− )-3-phosphoglycé rique disodium salt) Sigma-Aldrich Cat#P8877 Spermidine Sigma-Aldrich Cat#85558 Creatine phosphate crystalline, sup à 97% Sigma-Aldrich Cat#10621714001 PEG 8000 Sigma-Aldrich Cat#89510 Mg-glutamate Sigma-Aldrich Cat#49605 K-glutamate Alfa Aesar Cat#A17232 Amino acids - L-Alanine Sigma Cat#A7627 Amino acids - L-Arginine Sigma Cat#A5006 Amino acids - Glycine Sigma Cat#G7126 Amino acids - L-Histidine Merck Cat#1.04351.0100 Amino acids - L-Lysine Sigma Cat#L5626 Amino acids - L-Proline Sigma Cat#P0380 Amino acids - L-Serine Sigma Cat#S4500 Amino acids - L-Threonine Sigma Cat#T8625 Amino acids - L-Valine Sigma Cat#V0500 Amino acids - L-Aspartic Acid Sigma Cat#A9256 Amino acids - L-Asparagine anhydrous Sigma Cat#A0884 Amino acids - L-Cystine Sigma Cat#C8755 Amino acids - L-Glutamine Sigma Cat#G3126 Amino acids - L-Leucine Sigma Cat#L8000 Amino acids - L-Methionine Sigma Cat#64319 Amino acids - L-Tryptophan Sigma Cat#T8941 (Continued on next page) iScience 28, 113599, October 17, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Amino acids - L-Tyrosine Sigma Cat#T3754 Amino acids - L-Phenylalanine Sigma Cat#78019 Amino acids - lsoleucine Sigma Cat#I7403 BsmBI-v2 NEB Cat#R0739S BbsI NEB Cat#R0539S BsaI NEB Cat#R3733S DpnI NEB Cat#R0176L BamHI Promega Cat#R6021 EcoRV Promega Cat#R6351 T4 DNA ligase Promega Cat#M1801 T4 DNA ligase NEB CatM0202 Ampicillin Sigma–Aldrich Cat#A1593 (IPTG) isopropyl -d-1-thiogalactopyranoside Sigma–Aldrich Cat#I5502 Critical commercial assays Q5 High-Fidelity 2X Master Mix NEB Cat#M0492L PCR purification kit NEB Cat#T1130 NucleoBond Xtra Maxi kit Macherey-Nagel Cat#740414.1 Nano-Glo® HiBiT Lytic Detection System Promega Cat#N3030 Nano-Glo® HiBiT Lytic Detection System Promega Cat#N3040 Deposited data icfree-ml source code https://github.com/brsynth/icfree-ml https://doi.org/10.5281/zenodo.14900104 icfree-ml conda packages https://anaconda.org/bioconda/icfree-ml https://doi.org/10.5281/zenodo.14893904 icfree-ml Galaxy wrappers https://toolshed.g2.bx.psu.edu https://doi.org/10.5281/zenodo.14893963 AI-CellFree - Init Galaxy workflow https://galaxy-synbiocad.org/ u/joan/w/icfree-init https://doi.org/10.5281/zenodo.14901023 AI-CellFree - Core Galaxy workflow https://galaxy-synbiocad.org/ u/joan/w/icfree-core https://doi.org/10.5281/zenodo.14899706 Experimental models: Organisms/strains Escherichia coli DH5α Escherichia coli BL21 (DE3) Escherichia coli W3110 Oligonucleotides See Table S3 Recombinant DNA Colicin M Synthesized by GeneCust Colicin E1 Synthesized by GeneCust GFP1-10 Synthesized by GeneCust GFP11 Synthesized by GeneCust His-GFP1-10 This study His-GFP11-Colicin M This study His-GFP11-Colicin E1 This study His-HiBiT-Colicin M This study His-HiBiT-Colicin E1 This study His-HiBiT-sfGFP This study Software and algorithms Echo Cherry Pick software Beckman Coulter Protocol Designer Opentrons Image Lab TM Software Bio-Rad Laboratories ChatGPT-4 Openai e2 iScience 28, 113599, October 17, 2025 ..

Transfection:

Sterility:

cDNA Synthesis:

Article Title: ACTG1 facilitates proliferation and epithelial-mesenchymal transition-mediated metastasis via COL21A1 in gastric cancer
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-ACTG1 Abcam Cat#ab128898;RRID:AB11142942 Rabbit anti-GAPDH Abcam Cat#ab9485;RRID:AB307275 Rabbit anti- COL21A1 Immunoway Cat# YT5691;RRID:AB2889428 Rabbit anti- PCNA Proteintech Cat# 10205-2-AP;RRID:AB2160452 Rabbit anti- MMP9 WANLEI Cat# WL02435;N/A Rabbit anti- MMP2 WANLEI Cat# WL01246;N/A Rabbit anti- E-cadherin Proteintech Cat# 10205-2-AP;RRID:AB10694003 Rabbit anti- Vimentin CST Cat# D21H3;RRID:AB10695454 Critical commercial assays DMSO sigma Cat#D2650 Fetal bovine serum Gibco Cat#10099-141 RPMI-1640 Gibco Cat#11875093 Opti-MEM Invitrogen Cat#3198507 PrimeScriptTM II 1st Strand cDNA Synthesis Kit TAKARA Cat#6210A FastDigest BsmBI Fermentas Cat#FD0454 FastAP(Fermentas) Fermentas Cat#EF0654 10×FastDigest Buffer Fermentas Cat#B64 lentiCas9-Blast Addgene Cat#52962 Jo urn al Pr e-p roo f lentiGuide-Puro Addgene Cat#52963 Quick Ligase Buffer NEB Cat#B6058S Quick Ligase NEB Cat#M2200S T4 Ligation Buffer NEB Cat#B0202S T4 PNK NEB Cat#M0201S T4 DNA Ligase NEB CatM0202 Deposited data RNA-seq Data This paper HRA014677 Unedited images This paper MendeleyData: https://DOI:10.17632/pbv6cnhyhv.1 TCGA-STAD This paper GDC Experimental models: cell lines GES-1 CBCAS Cat# GNHu65 MKN-45 CBCAS Cat# TCHu280 AGS CBCAS Cat#TCHu232 HGC-27 CBCAS Cat# TCHu 22 293T CBCAS Cat# GNHu44 Experimental models: mouse 4-5 week old female athymic BALB/c-nu SiPeiFu Biotechnology Co., LTD 110324230101720362 Software and algorithms Graphpad Prism9.42 Dotmatics https://www.graphpad.com/ R-4.2.1 R https://www.r-project.org/ ..

Ligation:

RNA Sequencing:

Software:

Virus:

SYBR Green Assay:

Article Title: Donor transcription suppresses D-loops in cis and promotes genome stability.
Article Snippet: Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models W303 RAD5+ S. cerevisiae strains This study Table EV1 Recombinant DNA trp1::GAL-HO Pannunzio et al, DNA Rep 2008 https://doi.org/ 10.1016/ j.dnarep.2008.02.003 ura3::LY-HOcs Piazza et al, Cell 2017 Dataset EV1 ura3::L-HOcs This study Dataset EV1 ura3::L(reverse)-HOcs This study Dataset EV1 ura3::L-HOcs-Y This study Dataset EV1 lys2::LY Piazza et al, Cell 2017 Dataset EV1 Reagent/resource Reference or source Identifier or catalog number lys2::pDMC1-LY This study Dataset EV1 lys2::pTDH3-LY This study Dataset EV1 can1::LY Piazza et al, NCB 2021 Dataset EV1 can1::pDMC1-LY This study Dataset EV1 can1::pTDH3-LY This study Dataset EV1 lys2::L This study Dataset EV1 lys2::pDMC1-L This study Dataset EV1 lys2::pTDH3-L This study Dataset EV1 lys2::L(reverse) This study Dataset EV1 lys2::pDMC1-L(reverse) This study Dataset EV1 lys2::pTDH3-L(reverse) This study Dataset EV1 lys2::pGAL1-L This study Dataset EV1 lys2::pCUP1-L This study Dataset EV1 lys2::pTetO2-L This study Dataset EV1 lys2::S2 Piazza et al, Cell 2017 Dataset EV1 lys2::pDMC1-S2 This study Dataset EV1 lys2::pTDH3-S2 This study Dataset EV1 lys2::pGAL1-S2 This study Dataset EV1 ade2-I Ho et al, Mol Cell 2010 https://doi.org/ 10.1016/ j.molcel.2010.11.016 ade2-n Ho et al, Mol Cell 2010 Dataset EV1 pDMC1-ade2-n This study Dataset EV1 pTDH3-ade-n This study Dataset EV1 pYES2(empty) Thermofisher Sci. .. Dataset EV1; Cat# V825120 pYES2-RNH1 This study Dataset EV1 Antibodies Mouse anti-Rpb1 clone CTD4H8 Sigma-Aldrich Cat#05-623 Oligonucleotides and other sequence-based reagents PCR primers This study Table EV3 Chemicals, enzymes and other reagents Zymolyaze 100 T Carl Roth Cat#9329 Formaldehyde Sigma-Aldrich Cat#F8775 EcoRI-HF NEB Cat#R3101 HindIII-HF NEB Cat#R3104 T4 DNA ligase NEB CatM0202 Proteinase K NEB Cat# P8107S RNAseA EUROMEDEX Cat# 9707-C SSO Advanced Universal SYBR supermix Bio-Rad Cat#1725274 iTaqTM Universal SYBR® Green One-Step Kit Bio-Rad Cat#1725150 10 The EMBO Journal © The Author(s) D ow nloaded from https://w w w .em bopress.org on Septem ber 7, 2025 from IP 2405:4803:dadf:58d0:bc82:365e:71c7:1d84. .. Reagent/resource Reference or source Identifier or catalog number Qubit DNA high-sensitivity kit Thermo Fisher Scientific Cat#Q32851 Nucleospin RNA kit Machery Nagel Cat# MN06 740588.250 Dynabeads proteins G Thermo Fisher Scientific Cat#10003 Sodium DL-lactate Sigma-Aldrich Cat# L1375 Doxycycline Sigma-Aldrich Cat# D9891 Trioxsalen Sigma-Aldrich Cat# T6137 Software Prism 10 Graphpad https://www.gr aphpad.com/ CFX Maestro 2.0 Bio-Rad Biopython 1.78 Cock et al, Bioinformatics 2009 https:// biopython.org/ https://doi.org/ 10.1093/bioin formatics/btp163 Seaborn 0.12.2 Waskom JOSS 2021 https://seaborn.py data.org/ https://doi.org/ 10.21105/joss.03021.

Polymerase Chain Reaction:

Purification:

Gel Extraction:

Staining:

Cell Culture:

Luciferase:

Marker:

Plasmid Preparation:

DNA Purification:

Functional Assay:

Sequencing:

Synthesized:

other:

Article Title: Small-scale in situ Hi-C protocol for early embryos to resolve the three-dimensional genome structure.
Article Snippet: H 2 O Sigma Cat#S9638 KH 2 PO 4 Sigma Cat#P5655 NaHCO 3 Sigma Cat#S5761 MgCl 2 ⋅ 6H 2 O Sigma Cat#M2393 CaCl 2 ⋅ 2H 2 O Sigma Cat#C7902 Na-lactate 60 % (w/w) Sigma Cat#L7900 Fructose Sigma Cat#F3510 GSH Sigma Cat#G6013 D-penicillamine Sigma Cat#P4875 Hypotaurine Sigma Cat#H1384 Epinephrine Sigma Cat#E4250 Heparin Sigma Cat#H3149 HEPES Sigma Cat#4034 Hyaluronidase Sigma Cat#H3884 BO-IVC IVF Bioscience Cat#71005 Pronase E Sigma Cat#P8811 TrypLE Thermo Scientific Cat#12605010 Accutase Thermo Scientific Cat#A1110501 PBS Solarbio Cat#P1020 Recombinant albumin NEB Cat#B9200 NP-40 Beyotime Cat#ST2045 Proteinase inhibitor Roche Cat#4693132001 NEBuffer 2 NEB Cat#B7002S SDS BioFroxx Cat#3250GR500 Triton X-100 Sigma-Aldrich Cat#T8787 MboI NEB Cat#R0147S dCTP Invitrogen Cat#10217016 dGTP Invitrogen Cat#10218014 dTTP Invitrogen Cat#10219012 Nuclease-free water Invitrogen Cat#AM9932 Biotin-14-dATP Invitrogen Cat#19524016 DNA Polymerase I, Large (Klenow) Fragment NEB Cat#M0210S T4 DNA Ligase Reaction Buffer NEB Cat#B0202S T4 DNA Ligase NEB Cat#M0202S Proteinase K Roche Cat#3115887001 Phenol:chloroform:isoamyl alcohol mixture Solarbio Cat#P1012 Sodium acetate Thermo Scientific Cat#R1181 Alu I NEB Cat#R0137S rCutSmart Buffer NEB Cat#B6004V EDTA Beyotime Cat#ST066 Tris-HCl (pH 7.5) Beyotime Cat#ST775 Elution buffer QIAGEN Cat#1014609 DNA Marker TIANGEN Cat#4992937 E.Z.N.A.

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